B.A., University of Colorado Boulder, Biochemistry, 1994
B.A., University of Colorado Boulder, Molecular, Cellular and Developmental Biology, 1994
Mondays and Wednesdays, 10-11 a.m. and 1-2 p.m.; Tuesdays 2-3 p.m.
- Biochemistry I
- Biochemistry II
- Biological Principles
- Biological Principles Lab
- Cell Biology
- Cell Biology Enhancement
- Cell Biology Lab
- Chromosome Structures
- Genes in Society
- Genome Analysis
- Introduction to Organic Chemistry and Biochemistry
- Introduction to Organic Chemistry and Biochemistry Lab
- Living Planet
- Molecular Biology
- Nature of Science
- Organic Chemistry
- Scientific Writing
Using genetic and biochemical techniques, we examine the role chromatin (the heterogenous complex of DNA, RNA, and proteins that make up chromosomes) plays in centromere formation and gene expression in the simple eukaryotic model organism Neurospora crassa. To determine its role in centromere formation, we delete a centromere and examine neocentromeres that subsequently form. To determine its role in gene expression, we correlate protein binding in a gene’s promoter with the gene’s expression level in different genetic backgrounds that are missing chromatin factors or have modified forms of those factors.
Some of my research students are also examining the dot chromosome of various species of Drosophila fruit flies as part of the Genomics Education Partnership. They generate high quality finished sequences from raw sequence data and they annotate genes and other features to facilitate an evolutionary approach to understand the atypical chromatin strucutres and gene expression patterns of the dot chromosome.
- Hays SM, Swanson J, Selker EU. Identification and characterization of the genes encoding the core histones and histone variants of Neurospora crassa. Genetics. 2002 Mar;160(3):961-73.
- Hays SM and Selker EU. Making the selectable marker bar tighter and more economical. Fungal Genetics Newsletter. 2000 Aug;47:107.
- Klocko AD, Rountree MR, Grisafi PL, Hays SM, Adhvaryu KK, Selker EU. Neurospora importin α is required for normal heterochromatic formation and DNA methylation. PLoS Genetics. 2015 Mar;11(3):e1005083. doi:10.1371/journal.pgen.1005083